Isaac Scientific Publishing

Environmental Pollution and Protection

Microbial Treatment of Tannery Effluent by Augmenting Psychrotrophic Pseudomonas Putida Isolate

Download PDF (641.2 KB) PP. 23 - 39 Pub. Date: March 9, 2018

DOI: 10.22606/epp.2018.31003


  • Satyendra Kumar Garg*
    Centre of Excellence & DST-FIST supported Department of Microbiology, Dr. Ram Manohar Lohia Avadh University, Faizabad-224001, U.P., India
  • Siddhi Garg
    Wadia Institute of Himalayan Geology, Dehradun, Uttarakhand, India
  • Manikant Tripathi
    Centre of Excellence & DST-FIST supported Department of Microbiology, Dr. Ram Manohar Lohia Avadh University, Faizabad-224001, U.P., India
  • Kshitindra Singh
    Centre of Excellence & DST-FIST supported Department of Microbiology, Dr. Ram Manohar Lohia Avadh University, Faizabad-224001, U.P., India


In this study, Pseudomonas putida, a psychrotrophic isolate, has been employed for biological treatment of tannery effluent. The physicochemical parameters of tannery effluent were analysed. The dilution (1:1) of effluent, its supplementation with nutrients (e.g., glucose, yeast extract, peptone, each at 0.5%, w/v) and augmentation with P. putida test strain was done for bioremediation studies at natural pH 8.0. The efficient growth (OD 0.582), Cr6+ removal (61.0%), decolorization (60.1%) and dechlorination (87.5%) were observed in diluted effluent during 96-120 h incubation in shake flask trial. Box-Behnkem design suggested simultaneous dechlorination (95.8%) and Cr6+ removal (63.5%) in nutrients supplemented plus P. putida augmented only within 72 h incubation. The efficiency of bioremediation further enhanced in bioreactor. A direct correlation existed between chromate reductase activity and distribution of reduced chromium in different cell fractions and culture supernatant. Further enhanced dechlorination (68.2%) by 14.7% and Cr6+ removal (72.7%) by 6.5% employing immobilized co-culture (P. putida and Bacillus cereus) as compared to bioremediation by single immobilized P. putida biomass. High extent of effluent bioremediation by immobilized biomass offer an attractive future of P. putida biomass for eco-friendly in situ bioremediation of tannery wastewater.


Bioaugmentation, box behnkem design, immobilization, Psuedomonas putida, tannery effluent.


[1] O. P. Shukla, U. N. Rai, and S. Dubey, “Involvement and interaction of microbial communities in the transformation and stabilization of chromium during the composting of tannery effluent treated biomass of Vallisneria spiralis L., Bioresource Technology, vol. 100, pp. 2198-2203, 2009.

[2] S. Sharma, and P. Malaviya, “Bioremediation of tannery wastewater by chromium resistant fungal isolate Fusarium chlamydosporium SPFS2-g,” Current World Environment, vol. 9, pp. 721–727, 2014.

[3] S. Sharma, and P. Malaviya, “Bioremediation of tannery wastewater by Aspergillus flavus SPFT2,” International Journal of Current Microbiology and Applied Science, vol. 5, pp. 137-143, 2016.

[4] T. Srinath, T. Verma, P. W. Ramteke, and S. K. Garg, “Chromium (VI) biosorption and bioaccumulation by chromate resistant bacteria,” Chemosphere, vol. 48, pp. 427-435, 2002.

[5] W. H. Xu, Y. G. Liu, G. M. Zeng, X. Y. Li, H. X. Song, and Q. Q. Peng, “Characterization of Cr (VI) resistance and reduction by Pseudomonas aeruginosa,” Trans Nonferrous Metal Society China, vol. 19, pp. 1336-1341, 2009.

[6] R. Chandra, R. N. Bhargava, A. Kapley, and H.J. Purohit, “Bacterial diversity, organic pollutants and their metabolites in two aeration lagoons of common effluent treatment plant (CETP) during the degradation and detoxification of tannery wastewater,” Bioresource Technology, vol. 102, pp. 2333-2341, 2011.

[7] C. E. Paisio, M. A. Talano, P. S. Gonzalez, V. D. Busto, J. R. Talou, and E. Agostini, “Isolation and characterization of a Rhodococcus strain with phenol-degrading ability and its potential use for tannery effluent Biotreatment,” Environmental Science and Pollution Research, vol. 19, pp. 3430-3439, 2012.

[8] S. Sultan and S. Hasnain, “Chromium (VI) reduction by cell free extract of Ochrombactrum anthropi isolated from tannery effluent, Bulletin of Environmental Contamination and Toxicology, vol. 89, pp. 152-157, 2012.

[9] S. K. Garg, M. Tripathi, and T. Srinath, “Strategies for chromium bioremediation from tannery effluent,” Reviews of Environmental Contamination and Toxicology, vol. 217, pp. 75-140, 2012.

[10] M. Ilias, I. M. Rafiqullah, B. C. Debnath, K. S. B. Mannan, and M. M. Hoq, “Isolation and characterization of chromium (VI)-reducing bacteria from tannery effluents,” Indian Journal of Microbiology, vol. 51, pp. 76-81, 2011.

[11] M. M. Bradford, “A rapid and sensitive method for the quantification of micrograms quantities of protein utilizing the principle of protein dye binding,” Analytical Biochemistry, vol. 72, pp. 248-254, 1976.

[12] A. Lopez, N. Lazaro, and A. M. Marques, The interface technique: A simple method of cell immobilization in gel beads, Journal of Microbiological Methods, vol. 30, pp. 231-234, 1997.

[13] M. Tripathi and S. K. Garg, “Studies on selection of efficient bacterial strain simultaneously tolerant to hexavalent chromium and pentachlorophenol isolated from treated tannery effluent,” Research Journal of Microbiology, vol. 5, pp. 707-716, 2010.

[14] M. Tripathi, S. Vikram, R. K. Jain, and S. K. Garg, “Isolation and growth characteristics of chromium (VI) and pentachlorophenol tolerant bacterial isolate from treated tannery effluent for its possible use in simultaneous bioremediation,” Indian Journal of Microbiology, vol. 51, pp. 61-69, 2011.

[15] T. Srinath, S.K. Garg, and P.W. Ramteke, Biosorption and elution of chromium from immobilized Bacillus coagulans biomass, Indian Journal of Experimental Biology, vol. 4, pp. 986-990, 2003.

[16] C.P.P.A., “Technical Section Standard Method H5P,” Montreal, Canada: Canadian Pulp and Paper Association. 1974.

[17] M. Chowdhury, M. G. Mostafa, T.K. Biswas, and A. K. Saha, “Treatment of leather industrial effluents by filtration and coagulation process,” Water Resources and Industry, vol. 3, pp. 11-22, 2013.

[18] J. G. Bergmann and J. Sanik, “Determination of trace amounts of chlorine in naphtha” Analytical Chemistry, vol. 29, pp. 241-243, 1957.

[19] APHA, “Standard Methods for the Examination of Water and Wastewaters,” 20th ed, APHA, AWWA, WPCF, Washington DC, 1998.

[20] R. Steel and J. H. Torrie, “Principles and Procedures of Statistics,” McGraw Hill Book Co. Inc., New York. 1992.

[21] A. Bhattacharya, A. Gupta, A. Kaur, and D. Malik, “Simultaneous bioremediation of phenol and Cr(VI) from tannery wastewater using bacterial consortium,” International Journal of Applied Science and Biotechnology, vol. 3, pp. 50-55, 2015.

[22] O. O. Akpomie and B. O. Ejechi, “Bioremediation of soil contaminated with tannery effluent by combined treatment with cow dung and microorganisms isolated from tannery effluent, Journal of Bioremediation and Biodegradation, vol. 7, p. 354, 2016. doi: 10.4172/2155-6199.1000354

[23] C. K. Venil, V. Mohan, P. Lakshmanaperumalsamy, and M. B. Yerima, “Optimization of chromium removal by the indigenous bacterium Bacillus spp. REP02 using the response surface methodology,” ISRN Microbiology, vol. 9, 2011.

[24] S. K. Garg, M. Tripathi, S. K. Singh, and A. Singh, “Pentachlorophenol dechlorination and simultaneous Cr6+ reduction by Pseudomonas putida SKG-1 MTCC (10510): characterization of PCP dechlorination products, bacterial structure and functional groups,” Environmental Science and Pollution Research, vol. 20, pp. 2288–2304, 2013.

[25] A. Ganguli and A.K. Tripathi, Survival and chromate reducing ability of Pseudomonas aeruginosa in industrial effluents, Letters in Applied Microbiology, vol. 28, pp. 76-80, 1999.

[26] A. C. Poopal and R. S. Laxman, “Hexavalent chromate reduction by immobilized Streptomyces griseus,” Biotechnology Letters, vol. 30, pp. 1005-1010, 2008.

[27] A. Ganguli and A.K. Tripathi, “Bioremediation of toxic chromium from electroplating effluent by chromate-reducing Pseudomonas aeruginosa A2Chr in two bioreactors,” Applied Microbiology and Biotechnology, vol. 58, pp. 416-420, 2002.

[28] A.G. Murugesan and S. Maheswari, “Uptake of hexavalent chromium from aqueous solution employing live, dead and immobilized bacterial biomass,” Journal of Applied Science and Environmental Management, vol. 11, pp. 71-75, 2007.

[29] J. F. Benazir, R. Suganthi, D. Rajvel, M. P. Pooja, and B. Mathithumilan, “Bioremediation of chromium in tannery effluent by microbial consortia,” African Journal of Biotechnology, vol. 9, pp. 3140-3143, 2010.