Environmental Pollution and Protection

Microbial Treatment of Tannery Effluent by Augmenting Psychrotrophic Pseudomonas Putida Isolate

Download PDF (641.2 KB) PP. 23 - 39 Pub. Date: March 9, 2018

DOI: 10.22606/epp.2018.31003

Author(s)

Satyendra Kumar Garg^*
Centre of Excellence & DST-FIST supported Department of Microbiology, Dr. Ram Manohar Lohia Avadh University, Faizabad-224001, U.P., India
Siddhi Garg
Wadia Institute of Himalayan Geology, Dehradun, Uttarakhand, India
Manikant Tripathi
Centre of Excellence & DST-FIST supported Department of Microbiology, Dr. Ram Manohar Lohia Avadh University, Faizabad-224001, U.P., India
Kshitindra Singh
Centre of Excellence & DST-FIST supported Department of Microbiology, Dr. Ram Manohar Lohia Avadh University, Faizabad-224001, U.P., India

Abstract

In this study, Pseudomonas putida, a psychrotrophic isolate, has been employed for biological treatment of tannery effluent. The physicochemical parameters of tannery effluent were analysed. The dilution (1:1) of effluent, its supplementation with nutrients (e.g., glucose, yeast extract, peptone, each at 0.5%, w/v) and augmentation with P. putida test strain was done for bioremediation studies at natural pH 8.0. The efficient growth (OD 0.582), Cr6+ removal (61.0%), decolorization (60.1%) and dechlorination (87.5%) were observed in diluted effluent during 96-120 h incubation in shake flask trial. Box-Behnkem design suggested simultaneous dechlorination (95.8%) and Cr6+ removal (63.5%) in nutrients supplemented plus P. putida augmented only within 72 h incubation. The efficiency of bioremediation further enhanced in bioreactor. A direct correlation existed between chromate reductase activity and distribution of reduced chromium in different cell fractions and culture supernatant. Further enhanced dechlorination (68.2%) by 14.7% and Cr6+ removal (72.7%) by 6.5% employing immobilized co-culture (P. putida and Bacillus cereus) as compared to bioremediation by single immobilized P. putida biomass. High extent of effluent bioremediation by immobilized biomass offer an attractive future of P. putida biomass for eco-friendly in situ bioremediation of tannery wastewater.

Keywords

Bioaugmentation, box behnkem design, immobilization, Psuedomonas putida, tannery effluent.

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