Isaac Scientific Publishing

Modern Clinical Medicine Research

Modified Nanodiamonds as a New Carrier for Developing Reusable Enzymatic Test-Systems for Determination of Physiologically Important Substances

Download PDF (982.2 KB) PP. 7 - 17 Pub. Date: April 1, 2018

DOI: 10.22606/mcmr.2018.22001

Author(s)

  • Nikita Ronzhin*
    Institute of Biophysics, Federal Research Center “Krasnoyarsk Science Center of Siberian Branch of Russian Academy of Sciences”, Krasnoyarsk, Russia
  • Alexey Baron
    Institute of Biophysics, Federal Research Center “Krasnoyarsk Science Center of Siberian Branch of Russian Academy of Sciences”; Siberian Federal University, Krasnoyarsk, Russia
  • Alexey Puzyr
    Institute of Biophysics, Federal Research Center "Krasnoyarsk Science Center of Siberian Branch of Russian Academy of Sciences", Krasnoyarsk, Russia
  • Irina Baron
    Voino-Yasenetsky State Medical University, Krasnoyarsk, Russia
  • Andrey Burov
    Federal Research Center “Krasnoyarsk Science Center of Siberian Branch of Russian Academy of Sciences”; Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russia
  • Vladimir Bondar
    Institute of Biophysics, Federal Research Center "Krasnoyarsk Science Center of Siberian Branch of Russian Academy of Sciences", Krasnoyarsk, Russia

Abstract

The study demonstrates the application of detonation nanodiamonds in designing test-systems for determination of physiologically important blood substances. One (urease), two (glucose oxidase and peroxidase) and three (cholesterol esterase, cholesterol oxidase and peroxide) enzymes have been covalently immobilized onto the surface of modified nanodiamonds (MND) to develop test-systems for biochemical detection of urea, glucose, and total cholesterol, respectively. In all cases, the immobilized enzymes exhibit functional activity and catalyze the formation of a colored product in the presence of the analyte. It has been found that the obtained MND-enzyme(s) complexes effectively function in deionized water and different buffers providing a linear output of the final product over a wide range of temperatures, pH and analyte concentrations. The model biosensors were found to be suitable for repeated use and retain most of their activity after storage at 4 °C in deionized water for two months. Practical applicability of the glucose and total cholesterol biosensors is demonstrated in human serum samples.

Keywords

Biosensor, nanodiamonds, enzyme immobilization, glucose, cholesterol, urea.

References

[1] T. R. Silva and I. C. Vieira, “Biosensor based on gold nanoparticles stabilized in poly (allyamine hydrochloride) and decorated with laccase for determination of dopamine,” Analyst, vol. 141, no. 1, pp. 216–224, 2016.

[2] C. Sun, L. Gao, D. Wang, M. Zhang, Y. Liu, Z. Geng, W. Xu, F. Liu, H. Bian, “Biocompatible polypyrrole-block copolymer-gold nanoparticles platform for determination of inosine monophosphate with bi-enzyme biosensor,” Sensors and Actuators B: Chemical, vol. 230, pp. 521–527, 2016.

[3] O. O. Soldatkin, I. S. Kucherenko, V. M. Pyeshkova, A. L. Kukla, N. Jaffrezic-Renault, A. V. Elskaya, S. V. Dzyadevych, A. P. Soldatkin, “Novel conductometric biosensor based on three-enzyme system for selective determination of heavy metal ions,” Bioelectrochemistry, vol. 83, pp. 25–30, 2012.

[4] Z. Li, C. Xie, J. Wang, A. Meng, F. Zhang, “Direct electrochemistry of cholesterol oxidase immobilized on chitosan-graphene and cholesterol sensing,” Sensors and Actuators B: Chemical, vol. 208, pp. 505–511, 2015.

[5] R. Mundaca-Uribe, F. Bustos-Ramirez, C. Zaror-Zaror, M. Aranda-Bustos, J. Neira-Hinojosa, C. Pena-Farfal, “Development of a bienzymatic amperometric biosensor to determine uric acid in human serum, based on mesoporous silica (MCM-41) for enzyme immobilization,” Sensors and Actuators B: Chemical, vol. 195, pp. 58–62, 2014.

[6] L. Wang, X. Gao, L. Jin, Q. Wu, Z. Chen, X. Lin, “Amperometric glucose biosensor based on silver nanowires and glucose oxidase,” Sensors and Actuators B: Chemical, vol. 176, pp. 9–14, 2013.

[7] A. Boujakhrout, S. Jimenez-Falcao, P. Martinez-Ruiz, A. Sanchez, P. Diez, J.M. Pingarron, R. Villalonga, “Novel reduced graphene oxide-glycol chitosan nanohybrid for the assembly of amperometric enzyme biosensor for phenols,” Analyst, vol. 141, pp. 4162–4169, 2016.

[8] Z. Fan, Q. Lin, P. Gong, B. Liu, J. Wang, S. Yang, “A new enzymatic immobilization carrier based on graphene capsule for hydrogen peroxide biosensors,” Electrochimica Acta, vol. 151, pp. 186–194, 2015.

[9] T. Gessei, T. Arakawa, H. Kudo, H. Saito, K. Mitsubayashi, “Amperometric biosensor based on enzyme immobilization with post process for medical and multiple application,” Analytical Letters, vol. 47, pp. 1361–1374, 2014.

[10] P. Raghu, T. M. Reddy, K. Reddaiah, B. E. K. Swamy, M. Sreedhar, “Acetylcholinesterase based biosensor for monitoring of malathion and acephate in food samples: A voltammetric study,” Food Chemistry, vol. 142, pp. 188–196, 2014.

[11] I. M. Apetrei and C. Apetrei, “Amperometric biosensor based on polypyrrole and tyrosinase for the detection of tyramine in food samples,” Sensors and Actuators B: Chemical, vol. 178, pp. 40–46, 2013.

[12] D. Ling, N. Lee, T. Hyeon, “Chemical synthesis and assembly of uniformly sized iron oxide nanoparticles for medical applications,” Accounts of Chemical Research, vol. 48, no. 5, pp. 1276–1285, 2015.

[13] S. M. Davachi and B. Kaffashi, “Preparation and characterization of poly L-Lactide/Triclosan nanoparticles for specific antibacterial and medical application,” International Journal of Polymeric Materials and Polymeric Biomaterials, vol. 64, no. 10, pp. 497–508, 2015.

[14] Y. Wang, Q. Zhao, N. Han, L. Bai, J. Li, J. Liu, E. Che, L. Hu, Q. Zhang, T. Jiang, S. Wang, “Mesoporous silica nanoparticles in drug delivery and biomedical applications,” Nanomedicine: Nanotechnology, Biology and Medicine, vol. 11, no. 2, pp. 313–327, 2015.

[15] A. Gismondi, G. Reina, S. Orlanducci, F. Mizzoni, S. Gay, M. L. Terranova, A. Canini, “Nanodiamonds coupled with plant bioactive metabolites: A nanotech approach for cancer therapy,” Biomaterials, vol. 38, pp. 22–35, 2015.

[16] Y. Yang, A. M. Asiri, Z. Tang, D. Du, Y. Lin, “Graphene based materials for biomedical applications,” Materials Today, vol. 16, no. 10, pp. 365–373, 2013.

[17] L. Lai and A. S. Barnard, “Functionalized nanodiamonds for biological and medical applications,” Journal of Nanoscience and Nanotechnology, vol. 15, no. 2, pp. 989–999, 2015.

[18] G. Yesiller and M. K. Sezginturk, “A new methodology for the determination of enzyme activity based on carbon nanotubes and glucose oxidase,” Journal of Pharmaceutical and Biomedical Analysis, vol. 115, pp. 254–259, 2015.

[19] V. N. Mochalin, O. Shenderova, D. Ho, Y. Gogotsi, “The properties and applications of nanodiamonds,” Nature Nanotechnology, vol. 7, pp. 11–23, 2012.

[20] A. Krueger, “New carbon materials: biological applications of functionalized nanodiamond materials,” Chemistry - A European Journal, vol. 14, pp. 1382–1390, 2008.

[21] A. M. Schrand, S. A. C. Hens, O. A. Shenderova, “Nanodiamond particles: properties and perspectives for bioapplications,” Critical Reviews in Solid State and Materials Sciences, vol. 34, pp. 18–74, 2009.

[22] J. M. Say, C. van Vreden, D. J. Reilly, L. J. Brown, J. R. Rabeau, N. J. C. King, “Luminescent nanodiamonds for biomedical applications,” Biophysical Reviews, vol. 3, no. 4, pp. 171–184, 2011.

[23] D. Ho, C. H. K. Wang, E. K. H. Chow, “Nanodiamonds: The intersection of nanotechnology, drug development, and personalized medicine,” Science Advances, vol. 1, no. 7, pp. 1–14, 2015.

[24] V. S. Bondar and A. P. Puzyr, “Nanodiamonds for Biological Investigations,” Physics of the Solid State, vol. 46, no. 4, pp. 716–719, 2004.

[25] A. P. Puzyr, A. V. Baron, K. V. Purtov, E. V. Bortnikov, N. N. Skobelev, O. A. Mogilnaya, V. S. Bondar, “Nanodiamonds with novel properties: A biological study,” Diamond and Related Materials, vol. 16, no. 12, pp. 2124–2128, 2007.

[26] A. P. Puzyr, V. S. Bondar, A. A. Bukayemsky, G. E. Selyutin, V. F. Kargin, “Physical and chemical properties of modified nanodiamonds,” NATO Science Series II: Mathematics, Physics and Chemistry, vol. 192, pp. 261–270, 2005.

[27] N. Gibson, O. Shenderova, T. J. M. Luo, S. Moseenkov, V. Bondar, A. Puzyr, K. Purtov, Z. Fitzgerald, D. W. Brenner, “Colloidal stability of modified nanodiamond particles,” Diamond and Related Materials, vol. 18, pp. 620–626, 2009.

[28] A. P. Puzyr and V. S. Bondar, “Method of Production of Nanodiamonds of Explosive Synthesis with an Increased Colloidal Stability,” RU Patent No. 2252192, 2005.

[29] J. Brandt, L. O. Andersson, J. Porath, “Covalent attachment of proteins to polysaccharide carriers by means of benzoquinone,” Biochimica et Biophysica Acta, vol. 386, pp. 196–202, 1975.

[30] M. A. Mateescu, G. Weltrowska, E. Agostinelli, R. Saint-Andre, M. Weltrowski, B. Mondovi, “Ready to use p-benzoquinone-activated supports for biochemical coupling, with special applications for laccase immobilization,” Biotechnology Techniques, vol. 3, pp. 415–420, 1989.

[31] L. A. Osterman, Chromatography of Proteins and Nucleic Acids. Moscow: Nauka, 1985.

[32] A. N. Yeremin, T. V. Semashko, R. V. Mikhailova, “Cooxidation of phenol and 4-aminoantipyrin, catalyzed by polymers and copolymers of horseradish root peroxidase and Penicillium funiculosum 46.1 glucose oxidase,” Applied Microbiology and Biotechnology, vol. 42, no. 4, pp. 399–408, 2006.

[33] F. G. Menezes, A. C. O. Neves, D. F. Lima, S. D. Lourenco, L. C. Silva, K. M. G. Lima, “Bioorganic concepts involved in the determination of glucose, cholesterol and triglycerides in plasma using the enzymatic colorimetric method,” Química Nova, vol. 38, no.4, pp. 588–594, 2015.

[34] A. Tabacco, F. Meiattini, E. Moda, P. Tarli, “Simplified enzymic/colorimetric serum urea nitrogen determination,” Clinical Chemistry, vol. 25, no. 2, pp. 336–337, 1979.

[35] R. L. Searcy, J. E. Reardon, J. A. Foreman, “A new photometric method for serum urea nitrogen determination,” The American Journal of Medical Technology, vol. 33, no. 1, pp. 15–20, 1967.