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Journal of Current Microbiology
JCMb > Volume 1, Number 1, October 2018

Diauxic Growth Pattern in Thermophilic Bacillus spp with Respect to Production of Thermostable Amylase

Download PDF  (1137.9 KB)PP. 15-27,  Pub. Date:October 23, 2018


Author(s)
Ashish Dhyani, Rahul Jain, Anita Pandey, Avinash Sharma, Kusum Dhakar, Veena Pande
Affiliation(s)
Biotechnological Applications, G.B. Pant National Institute of Himalayan Environment and Sustainable Development, Kosi-Katarmal, Almora – 263 643, Uttarakhand, India
Biotechnological Applications, G.B. Pant National Institute of Himalayan Environment and Sustainable Development, Kosi-Katarmal, Almora – 263 643, Uttarakhand, India
Biotechnological Applications, G.B. Pant National Institute of Himalayan Environment and Sustainable Development, Kosi-Katarmal, Almora – 263 643, Uttarakhand, India
Microbial Culture Collection, National Center for Cell Sciences, Ganeshkhind, Pune 411 007, Maharashtra, India
Biotechnological Applications, G.B. Pant National Institute of Himalayan Environment and Sustainable Development, Kosi-Katarmal, Almora – 263 643, Uttarakhand, India
Department of Biotechnology, Kumaun University, Bhimtal Campus, Bhimtal– 263 136, Uttarakhand, India
Abstract
Thermophilc bacteria are emerging as source of biotechnological applications in recent years. The present study is focused on the growth patterns of thermophilic bacteria, isolated from the Soldhar hot spring (Indian Himalaya), with respect to amylase production. The morphologically and physiologically different bacterial isolates viz. GBPI_30, GBPI_31 and GBPI_35, identified using 16S rDNA gene sequencing, showed maximum similarity with Bacillus licheniformis, B. subtilis and B. licheniformis. These isolates were able to grow in wide temperature 25 to 85 ℃ (optimum 55 ℃) as well as wide pH (5-14) range. While the bacterial isolates followed normal growth pattern in Tryptone Yeast extract broth, they exhibited diauxic growth pattern with respect to amylase production in starch broth. Maximum amylase production was recorded during the stationary phase of growth at different temperatures i.e. 65 ℃, 35 ℃ and 55 ℃ for GBPI_30, GBPI_31, and GBPI_35, respectively. Amylases of the bacterial isolates showed different temperature optima for enzyme activity i.e. 40 ℃, 60 ℃ and 50 ℃, while pH optima was recorded similar for all the three isolates. Amylase production in variable temperature conditions by thermophilic Bacillus spp. would be advantageous for their survival in natural habitat along with the associated biotechnological applications.
Keywords
Thermophiles, Bacillus, growth curve, amylase, hot spring, Indian Himalayan region
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